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GenScript corporation
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The protein encoded by this gene interacts with the dedicator of cyto kinesis 1 protein Similarity to a C elegans protein suggests that this protein may function in phagocytosis of apoptotic cells and in cell
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ELMO2 707 720 goat polyclonal antibody Aff Purified
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Additional name(s) for this target protein: Engulfment and cell motility 2
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Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small
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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: The Atypical Guanine Nucleotide Exchange Factor Dock4 Regulates Neurite Differentiation through Modulation of Rac1 GTPase and Actin Dynamics
doi: 10.1074/jbc.m113.458612
Figure Lengend Snippet: FIGURE 6. The SH3-mediated interaction is important for Dock4-regulated neurite outgrowth. A, two N-terminal fragments of Dock4, SH3 (amino acids (aa) 1–161) and SH3-L (amino acids 1–417), were constructed as depicted. B, the expression of Dock4-SH3 and SH3-L were confirmed in HEK293T cells by overexpression. C, ELMO2 and HA-tagged Dock4-FL or SH3 were cotransfected into HEK293T cells. The cell lysates were immunoprecipitated (IP) with HA antibody,followedbyimmunoblottingusingELMO2antibody.D,ELMO2andHA-taggedDock4-SH3andSH3-LwerecotransfectedintoHEK293Tcells.Thecell lysates were immunoprecipitated with HA antibody, followed by immunoblotting using ELMO2 antibody. E, Neuro-2a cells were cotransfected with GFP and various plasmids as indicated, followed by treatment with RA for 2 days. Cells were immunostained using -tubulin III antibody for visualization of neurites. Scale bar 50 m. Average length of the longest neurite (F) and average length of total neurites (G) were measured. *, p 0.05; **, p 0.01; ***, p 0.001; Student’s t test. At least 40 cells/group were analyzed in each experiment. n 3. Error bars depict mean S.E.
Article Snippet: Primary antibodies against Dock4,
Techniques: Construct, Expressing, Over Expression, Immunoprecipitation, Western Blot